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Description
UniOne® TR-FRET His Check kitProduct Specification Host Human Stability & Storage 80 Background The kit employs homogeneous time resolved fluorescence (TR FRET) technology for the detection of His tagged proteins. It can be used to confirm whether the His tag motif of a protein is recognizable by anti His antibodies or to estimate the concentration of His tagged proteins. As illustrated, the His tagged protein is labeled with an Acceptor (Ac Protein His Tag), while the anti His
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
The kit employs homogeneous time-resolved fluorescence (TR-FRET) technology for the detection of His-tagged proteins. It can be used to confirm whether the His-tag motif of a protein is recognizable by anti-His antibodies or to estimate the concentration of His-tagged proteins.
As illustrated, the His-tagged protein is labeled with an Acceptor (Ac-Protein His Tag), while the anti-His antibody is labeled with Europium (Eu-anti-His). Upon binding of the antibody to the His-tag on the protein, the proximity between the donor and acceptor enables fluorescence resonance energy transfer (FRET), resulting in a specific 665 nm emission signal from the acceptor. In the presence of other His-tagged proteins, they compete with Ac-Protein His Tag for binding to Eu-anti-His, thereby reducing FRET signal generation. Higher concentrations of competing His-tagged proteins lead to stronger competition and lower signals. The signal intensity is proportional to the binding between Ac-Protein His Tag and Eu-anti-His, with no washing steps required.
Components
Components |
Concentration |
100T |
500T |
2500T |
10000T |
Storage Temperature |
His Tag Control |
500μM |
10μL |
20μL |
100μL |
400μL |
-80℃ |
Eu-anti-His |
100 × |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Ac-Protein His Tag |
100× |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Detection buffer |
10 × |
400μL |
2mL |
10mL |
40mL |
-80℃ |
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
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